FastQCFastQC Report
Sat 4 Jun 2022
EGAF00005441227

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005441227
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences49391335
Sequences flagged as poor quality0
Sequence length151
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCGTTTCCGACCTGGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGGT982030.19882637308750614No Hit
ATCGGAAGAGCACACGTCTGAACTCCAGTCACGCGCAAGCATCTCGTATG930090.18831035848696942TruSeq Adapter, Index 16 (97% over 35bp)
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA731910.14818591155715877No Hit
CCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGATG575770.11657307906336202No Hit
CCCGTCCCGGGGGTTGGCCGCGCGGGCCCCGGTGGGGCGGCCACCCGGGG548650.1110822374005481No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)