FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005441247

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005441247
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21570235
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA241510.1119644732660539No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATCGCG10700.046.7482389
GCGTACG15550.039.6354752
CGTACGG16900.036.891883
ACCGCGT17050.031.8886996
CCGCGTT19250.027.8676857
GGCGTAC24400.027.0345571
GACCGCG21250.025.585995
CGCGTTC23200.023.747928
TCGACCC34500.023.325073
GACCCGT35900.023.0202665
GTACGGA28900.021.8233454
GTTCGAT39550.020.9410511
AGACCGC26150.020.7916774
ATATCGC25550.020.4287368
CGACCCG43700.018.9113854
GTCGCAT12900.018.181381
GATATCG28900.018.06077
GTTACGG45800.017.7277037
GTCCGGT17000.017.6767121
GTGTCGA24900.017.1772084
Produced by FastQC (version 0.11.5)