FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005441248

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005441248
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21570235
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTCGAC63150.028.8366071
CCGTGCG63750.028.640098
TCGACCC67650.027.9526333
CGTGCGG64800.027.8405869
ACGTGCG41400.027.475998
GTCGACC69000.026.155342
TATCGCG19150.025.7272999
ACCCGTG79450.023.7101046
CGACCCG82700.022.5152424
CGTGCGC52350.021.8672649
AGGTCGA51800.021.5399656
TAACACG65900.017.5907174
GACCCGT116250.017.014485
GTCTAAC84500.016.1411911
AACACGT75000.015.2631745
ATATCGC33400.015.184688
GGCGCGT20450.014.8800714
GGTCGCC577950.014.115626145
CCGTATC352300.014.046241145
CGCCGTA414300.014.00779145
Produced by FastQC (version 0.11.5)