FastQCFastQC Report
Sat 4 Jun 2022
EGAF00005441274

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005441274
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences42392421
Sequences flagged as poor quality0
Sequence length151
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTCGACCCGTGCGGAGGAGCGAGGAGGAAGGACGCGCGAGGGCCGGGAC2061070.48618832125676426No Hit
ATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTTTGGACTCGTGTAGATCTC1468190.3463331334627008Illumina Single End PCR Primer 1 (96% over 31bp)
GTCGACCCGTGCGGAGGAGCGAGGAGGAAGGACGCGCGAGGGCCGGGACC1161350.2739522708552078No Hit
GCTGCAGGTCGACCCGTGCGGAGGAGCGAGGAGGAAGGACGCGCGAGGGC1126740.2657880756562594No Hit
CTGCAGGTCGACCCGTGCGGAGGAGCGAGGAGGAAGGACGCGCGAGGGCC699790.1650743183551607No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA569510.1343424099321905No Hit
CAGGTCGACCCGTGCGGAGGAGCGAGGAGGAAGGACGCGCGAGGGCCGGG473730.1117487486737311No Hit
CCCGTCCCGGGGGTTGGCCGCGCGGGCCCCGGTGGGGCGGCCACCCGGGG460170.10855006370124509No Hit
GCAGGTCGACCCGTGCGGAGGAGCGAGGAGGAAGGACGCGCGAGGGCCGG440210.10384167490693678No Hit
CCGGGCCCGGCGCGCCGGGGGCCGCTACCGGCCTCACACCGTCCACGGGC432840.10210315659961954No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)