FastQCFastQC Report
Sat 4 Jun 2022
EGAF00005441294

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005441294
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences44356905
Sequences flagged as poor quality0
Sequence length151
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTCGACCCGTGCGGAGGAGCGAGGAGGAAGGACGCGCGAGGGCCGGGAC2465030.5557263294181594No Hit
GTCGACCCGTGCGGAGGAGCGAGGAGGAAGGACGCGCGAGGGCCGGGACC1340320.3021671597691498No Hit
GCTGCAGGTCGACCCGTGCGGAGGAGCGAGGAGGAAGGACGCGCGAGGGC1294380.2918102604318313No Hit
CTGCAGGTCGACCCGTGCGGAGGAGCGAGGAGGAAGGACGCGCGAGGGCC924780.2084861421237573No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA673060.15173736760939477No Hit
CAGGTCGACCCGTGCGGAGGAGCGAGGAGGAAGGACGCGCGAGGGCCGGG555080.12513947941137912No Hit
CCCGTGCGGAGGAGCGAGGAGGAAGGACGCGCGAGGGCCGGGACCCCGGG528750.11920353775810102No Hit
CCCGTCCCGGGGGTTGGCCGCGCGGGCCCCGGTGGGGCGGCCACCCGGGG528120.11906150801098499No Hit
ATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAACAGGTTGTGTAGATCTC495460.11169850556525529Illumina Single End PCR Primer 1 (96% over 32bp)
TGCAGGTCGACCCGTGCGGAGGAGCGAGGAGGAAGGACGCGCGAGGGCCG486690.10972136130778286No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)