FastQCFastQC Report
Tue 14 May 2024
EGAF00005441340

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005441340
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences46769767
Total Bases7 Gbp
Sequences flagged as poor quality0
Sequence length151
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTTAAGTGGTGTGTAGATCTC2134640.4564145038396279Illumina Single End Sequencing Primer (100% over 31bp)
GGTCGACCCGTGCGGAGGAGCGAGGAGGAAGGACGCGCGAGGGCCGGGAC1029930.22021277121179586No Hit
GTCTAACACGTGCGCGAGTCGGGGGCTCGCACGAAAGCCGCCGTGGCGCA797490.17051399892584457No Hit
GTCGACCCGTGCGGAGGAGCGAGGAGGAAGGACGCGCGAGGGCCGGGACC631830.13509368135188699No Hit
GCTGCAGGTCGACCCGTGCGGAGGAGCGAGGAGGAAGGACGCGCGAGGGC556350.11895505059924716No Hit
GTTCTGGGCTGTAGTGCGCTATGCCGATCGGGTGTCCGCACTAAGTTCGG514390.10998344293654488No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)