FastQCFastQC Report
Tue 28 Jun 2022
EGAF00005441341

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005441341
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences45810549
Sequences flagged as poor quality0
Sequence length151
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1901310.4150375931971477No Hit
CCCGTCCCGGGGGTTGGCCGCGCGGGCCCCGGTGGGGCGGCCACCCGGGG1685380.36790216157418243No Hit
GGTCGACCCGTGCGGAGGAGCGAGGAGGAAGGACGCGCGAGGGCCGGGAC1149810.25099240788404437No Hit
GTCCCCGTCCCGGGGGTTGGCCGCGCGGGCCCCGGTGGGGCGGCCACCCG1078540.23543485584510243No Hit
GTCGACCCGTGCGGAGGAGCGAGGAGGAAGGACGCGCGAGGGCCGGGACC1068540.23325195251425604No Hit
ATCGGAAGAGCACACGTCTGAACTCCAGTCACCCGTGAAGATCTCGTATG914790.1996898138024934TruSeq Adapter, Index 16 (97% over 36bp)
GCTGCAGGTCGACCCGTGCGGAGGAGCGAGGAGGAAGGACGCGCGAGGGC565490.12344100045603033No Hit
CCGGGGGTTGGCCGCGCGGGCCCCGGTGGGGCGGCCACCCGGGGTCCCGG558090.12182565199120403No Hit
CCCGCCGGTCCCCGTCCCGGGGGTTGGCCGCGCGGGCCCCGGTGGGGCGG552730.12065561580587038No Hit
GGCGTACGGAAGACCCGCTCCCCGGCGCCGCTCGTGGGGGGCCCAAGTCC499050.10893779072588716No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)