FastQCFastQC Report
Sat 4 Jun 2022
EGAF00005441343

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005441343
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences53698307
Sequences flagged as poor quality0
Sequence length151
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGGAAGAGCACACGTCTGAACTCCAGTCACCAACAATGATCTCGTATG6686531.2452031308920037TruSeq Adapter, Index 23 (97% over 36bp)
GTTGATTTCTGTTCCTGCAGCTACTTAGATGTTTCAGTTCACTGCGTTCG1260100.23466289169973273No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1047710.19511043430102926No Hit
CCCGTCCCGGGGGTTGGCCGCGCGGGCCCCGGTGGGGCGGCCACCCGGGG758130.14118322203342462No Hit
CTCCGTTTCCGACCTGGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGGT654320.12185114141494255No Hit
CTTGGTTTCGGGTCTACACCCAGCGACTGAACGCCCTATTCGGACTCGCT634640.11818622140172873No Hit
GGTCGACCCGTGCGGAGGAGCGAGGAGGAAGGACGCGCGAGGGCCGGGAC631510.11760333524109055No Hit
GTCAGCATTCGCACTTCTGATACCTCCAGCATCCTTTACAAGACACCTTC592880.110409439910275No Hit
CCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGATG589880.10985076307899241No Hit
GTCGACCCGTGCGGAGGAGCGAGGAGGAAGGACGCGCGAGGGCCGGGACC558990.10409825397288597No Hit
GTGGCTATTCACAGGCGCGATCCCACTACTGATCAGCACGGGAGTTTTGA544450.10139053359726966No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)