FastQCFastQC Report
Fri 27 May 2022
EGAF00005441344

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005441344
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences53698307
Sequences flagged as poor quality0
Sequence length151
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTCCGTGAAGGTGTAGATCTC6571041.223695935143728Illumina Single End PCR Primer 1 (96% over 31bp)
GGTCGACCCGTGCGGAGGAGCGAGGAGGAAGGACGCGCGAGGGCCGGGAC1464250.27268085006851334No Hit
GTTTGATCCTGGCTCAGATTGAACGCTGGCGGCATGCCTTACACATGCAA1344870.2504492366956746No Hit
GTCGACCCGTGCGGAGGAGCGAGGAGGAAGGACGCGCGAGGGCCGGGACC906320.16877999524268056No Hit
GCTGCAGGTCGACCCGTGCGGAGGAGCGAGGAGGAAGGACGCGCGAGGGC761530.14181638910887825No Hit
GTGCATGTGGTGGATGCCTTGGCGATCACAGGCGATGAAGGACGTAGTAG601220.11196256150124062No Hit
AGTGCATGTGGTGGATGCCTTGGCGATCACAGGCGATGAAGGACGTAGTA538840.10034580792277119No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)