FastQCFastQC Report
Sat 4 Jun 2022
EGAF00005441345

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005441345
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences48352826
Sequences flagged as poor quality0
Sequence length151
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGGAAGAGCACACGTCTGAACTCCAGTCACGGTGAACCATCTCGTATG3761900.7780103690319983TruSeq Adapter, Index 9 (97% over 35bp)
CCCGTCCCGGGGGTTGGCCGCGCGGGCCCCGGTGGGGCGGCCACCCGGGG696600.14406603659525505No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA642080.13279058394642745No Hit
GGTCGACCCGTGCGGAGGAGCGAGGAGGAAGGACGCGCGAGGGCCGGGAC490250.1013901441872291No Hit
GTCGACCCGTGCGGAGGAGCGAGGAGGAAGGACGCGCGAGGGCCGGGACC490230.10138600792433518No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)