FastQCFastQC Report
Fri 1 Jul 2022
EGAF00005441348

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005441348
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences38372071
Sequences flagged as poor quality0
Sequence length151
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTCTGCTTCCGTGTAGATCTC852720.22222412754318108Illumina Single End PCR Primer 1 (96% over 31bp)
GTCTAACACGTGCGCGAGTCGGGGGCTCGCACGAAAGCCGCCGTGGCGCA850860.22173939999224956No Hit
GGTCGACCCGTGCGGAGGAGCGAGGAGGAAGGACGCGCGAGGGCCGGGAC740380.19294762589175862No Hit
GTCGACCCGTGCGGAGGAGCGAGGAGGAAGGACGCGCGAGGGCCGGGACC435500.11349400453261958No Hit
GGAGTCTAACACGTGCGCGAGTCGGGGGCTCGCACGAAAGCCGCCGTGGC400490.1043701811142797No Hit
CGTCCCCCGACCGGCGACCGGCCGCCGCCGGGCGCATTTCCACCGCGGCG383910.10004933014952463No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)