FastQCFastQC Report
Sat 4 Jun 2022
EGAF00005441349

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005441349
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences44817072
Sequences flagged as poor quality0
Sequence length151
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGGAAGAGCACACGTCTGAACTCCAGTCACCTACGACAATCTCGTATG2548400.5686225998878285TruSeq Adapter, Index 23 (97% over 36bp)
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1531010.3416131245700299No Hit
CCCGTCCCGGGGGTTGGCCGCGCGGGCCCCGGTGGGGCGGCCACCCGGGG831120.1854471885178041No Hit
GGTCGACCCGTGCGGAGGAGCGAGGAGGAAGGACGCGCGAGGGCCGGGAC647690.14451858880919305No Hit
GTCGACCCGTGCGGAGGAGCGAGGAGGAAGGACGCGCGAGGGCCGGGACC596840.13317246606382496No Hit
GTCCCCGTCCCGGGGGTTGGCCGCGCGGGCCCCGGTGGGGCGGCCACCCG565620.12620637064375825No Hit
CCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGATG449300.10025197540794274No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)