FastQCFastQC Report
Sat 4 Jun 2022
EGAF00005441353

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005441353
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences49021446
Sequences flagged as poor quality0
Sequence length151
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGGAAGAGCACACGTCTGAACTCCAGTCACTTACAGGAATCTCGTATG1039730.2120969667031038TruSeq Adapter, Index 4 (97% over 36bp)
CTCCGTTTCCGACCTGGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGGT534600.10905431063783799No Hit
CCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGATG516210.1053028913100605No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)