FastQCFastQC Report
Sat 4 Jun 2022
EGAF00005441356

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005441356
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences39900791
Sequences flagged as poor quality0
Sequence length151
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTCGACCCGTGCGGAGGAGCGAGGAGGAAGGACGCGCGAGGGCCGGGAC1703800.4270090785919507No Hit
GTCGACCCGTGCGGAGGAGCGAGGAGGAAGGACGCGCGAGGGCCGGGACC835010.20927154050655286No Hit
GCTGCAGGTCGACCCGTGCGGAGGAGCGAGGAGGAAGGACGCGCGAGGGC771750.19341721822005983No Hit
CTGCAGGTCGACCCGTGCGGAGGAGCGAGGAGGAAGGACGCGCGAGGGCC571820.14331044214136004No Hit
GTCTAACACGTGCGCGAGTCGGGGGCTCGCACGAAAGCCGCCGTGGCGCA569490.14272649381813007No Hit
ATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTTTATAACCGTGTAGATCTC491660.12322061484946502Illumina Single End PCR Primer 1 (96% over 31bp)
CGTCCCCCGACCGGCGACCGGCCGCCGCCGGGCGCATTTCCACCGCGGCG424170.10630616320363173No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)