FastQCFastQC Report
Sat 4 Jun 2022
EGAF00005441360

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005441360
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences44626778
Sequences flagged as poor quality0
Sequence length151
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAACGTTCCGTGTAGATCTC2484950.5568293547878361Illumina Single End PCR Primer 1 (96% over 32bp)
GGTCGACCCGTGCGGAGGAGCGAGGAGGAAGGACGCGCGAGGGCCGGGAC685580.1536252516370328No Hit
GTCTAACACGTGCGCGAGTCGGGGGCTCGCACGAAAGCCGCCGTGGCGCA555090.12438496007934968No Hit
GTTCTGGGCTGTAGTGCGCTATGCCGATCGGGTGTCCGCACTAAGTTCGG474270.10627475727689774No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)