FastQCFastQC Report
Sat 4 Jun 2022
EGAF00005441363

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005441363
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences51395553
Sequences flagged as poor quality0
Sequence length151
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGGAAGAGCACACGTCTGAACTCCAGTCACAGGCAGAGATCTCGTATG4626440.9001634830157387TruSeq Adapter, Index 13 (97% over 37bp)
CCCCGAGCCACCTTCCCCGCCGGGCCTTCCCAGCCGTCCCGGAGCCGGTC648200.1261198609926427No Hit
CTCCGTTTCCGACCTGGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGGT546780.10638663621344827No Hit
GTCGCGCCGTGGGAGGGGTGGCCCGGCCCCCCCACGAGGAGACGCCGGCG536560.10439813732522733No Hit
CCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGATG529800.1030828484324315No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)