FastQCFastQC Report
Thu 16 Jun 2022
EGAF00005441368

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005441368
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences46423399
Sequences flagged as poor quality0
Sequence length151
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGTACTCCGTGTAGATCTC3902530.8406385753873816Illumina Single End PCR Primer 1 (96% over 33bp)
GGTCGACCCGTGCGGAGGAGCGAGGAGGAAGGACGCGCGAGGGCCGGGAC1922830.41419414377650376No Hit
GTCGACCCGTGCGGAGGAGCGAGGAGGAAGGACGCGCGAGGGCCGGGACC1199710.25842786737782814No Hit
GCTGCAGGTCGACCCGTGCGGAGGAGCGAGGAGGAAGGACGCGCGAGGGC983240.21179836487199055No Hit
CTGCAGGTCGACCCGTGCGGAGGAGCGAGGAGGAAGGACGCGCGAGGGCC561070.12085931062479936No Hit
CCCGTCCCGGGGGTTGGCCGCGCGGGCCCCGGTGGGGCGGCCACCCGGGG480950.10360077253283415No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)