FastQCFastQC Report
Thu 16 Jun 2022
EGAF00005441375

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005441375
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences47464139
Sequences flagged as poor quality0
Sequence length151
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGGAAGAGCACACGTCTGAACTCCAGTCACACACTAAGATCTCGTATG1457140.30699808965248476TruSeq Adapter, Index 5 (97% over 37bp)
CCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGATG762920.16073608751230062No Hit
GCCCTCTTGAACTCTCTCTTCAAAGTTCTTTTCAACTTTCCCTTACGGTA616790.12994863343038837No Hit
CTCCGTTTCCGACCTGGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGGT567740.11961451570837511No Hit
GTGGCTATTCACAGGCGCGATCCCACTACTGATCAGCACGGGAGTTTTGA529380.11153262466216862No Hit
GCTCCGTTTCCGACCTGGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGG504900.1063750466431088No Hit
GTTGATTTCTGTTCCTGCAGCTACTTAGATGTTTCAGTTCACTGCGTTCG501050.10556390794321582No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)