FastQCFastQC Report
Sat 4 Jun 2022
EGAF00005441376

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005441376
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences47464139
Sequences flagged as poor quality0
Sequence length151
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTATATGGATGTGTAGATCTC1364700.2875223334399893Illumina Single End PCR Primer 1 (97% over 35bp)
GTCTAACACGTGCGCGAGTCGGGGGCTCGCACGAAAGCCGCCGTGGCGCA683330.14396763838905832No Hit
GTTCTGGGCTGTAGTGCGCTATGCCGATCGGGTGTCCGCACTAAGTTCGG509540.1073526267905123No Hit
GGTCGACCCGTGCGGAGGAGCGAGGAGGAAGGACGCGCGAGGGCCGGGAC491810.10361717506347265No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)