FastQCFastQC Report
Thu 16 Jun 2022
EGAF00005441379

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005441379
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences47659116
Sequences flagged as poor quality0
Sequence length151
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2425850.5090002089002238No Hit
ATCGGAAGAGCACACGTCTGAACTCCAGTCACTGGTGGCAATCTCGTATG2315010.48574337803495976TruSeq Adapter, Index 10 (97% over 35bp)
GCCCTCTTGAACTCTCTCTTCAAAGTTCTTTTCAACTTTCCCTTACGGTA1167560.2449814637770453No Hit
CTCTTAACGGTTTCACGCCCTCTTGAACTCTCTCTTCAAAGTTCTTTTCA877970.1842186917608795No Hit
CTCTCTTCAAAGTTCTTTTCAACTTTCCCTTACGGTACTTGTTGACTATC773010.162195622763964No Hit
CTTCAAAGTTCTTTTCAACTTTCCCTTACGGTACTTGTTGACTATCGGTC578310.1213429976334433No Hit
GTTCGATTAGTCTTTCGCCCCTATACCCAGGTCGGACGACCGATTTGCAC549410.1152791000151996No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)