FastQCFastQC Report
Sat 4 Jun 2022
EGAF00005441380

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005441380
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences47659116
Sequences flagged as poor quality0
Sequence length151
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTTTACAGGAGTGTAGATCTC2296070.48176932194881666Illumina Single End PCR Primer 1 (96% over 31bp)
GTCTAACACGTGCGCGAGTCGGGGGCTCGCACGAAAGCCGCCGTGGCGCA1091820.22908943590141287No Hit
GGTCGACCCGTGCGGAGGAGCGAGGAGGAAGGACGCGCGAGGGCCGGGAC635850.1334162387737112No Hit
GGAGTCTAACACGTGCGCGAGTCGGGGGCTCGCACGAAAGCCGCCGTGGC633680.1329609218937254No Hit
GTGTGAGGCCGGTAGCGGCCCCCGGCGCGCCGGGCCCGGGTCTTCCCGGA553430.11612259027213177No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)