FastQCFastQC Report
Thu 12 May 2022
EGAF00005441383

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005441383
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences42807741
Sequences flagged as poor quality0
Sequence length151
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGGAAGAGCACACGTCTGAACTCCAGTCACGCTTGTCAATCTCGTATG2705360.6319791553588403TruSeq Adapter, Index 8 (97% over 37bp)
CCCGTCCCGGGGGTTGGCCGCGCGGGCCCCGGTGGGGCGGCCACCCGGGG481770.11254272912929464No Hit
CTCCGTTTCCGACCTGGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGGT444840.1039157847642556No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)