FastQCFastQC Report
Sat 4 Jun 2022
EGAF00005441384

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005441384
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences42807741
Sequences flagged as poor quality0
Sequence length151
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTGTATGTTCGTGTAGATCTC2471700.5773955696470879Illumina Single End PCR Primer 1 (96% over 31bp)
GGTCGACCCGTGCGGAGGAGCGAGGAGGAAGGACGCGCGAGGGCCGGGAC883690.20643228989822193No Hit
GTCGACCCGTGCGGAGGAGCGAGGAGGAAGGACGCGCGAGGGCCGGGACC522890.12214846842770798No Hit
GTCTAACACGTGCGCGAGTCGGGGGCTCGCACGAAAGCCGCCGTGGCGCA486140.11356357253236044No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)