FastQCFastQC Report
Wed 15 May 2024
EGAF00005441396

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005441396
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences47309955
Total Bases7.1 Gbp
Sequences flagged as poor quality0
Sequence length151
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTGCAGAATTGTGTAGATCTC2511330.5308248549380358Illumina Single End Sequencing Primer (100% over 31bp)
GGTCGACCCGTGCGGAGGAGCGAGGAGGAAGGACGCGCGAGGGCCGGGAC1925510.4069989075237971No Hit
GTCGACCCGTGCGGAGGAGCGAGGAGGAAGGACGCGCGAGGGCCGGGACC1077200.22768992276572655No Hit
GCTGCAGGTCGACCCGTGCGGAGGAGCGAGGAGGAAGGACGCGCGAGGGC972370.20553179558086668No Hit
CTGCAGGTCGACCCGTGCGGAGGAGCGAGGAGGAAGGACGCGCGAGGGCC528880.11179042550346961No Hit
CGGGGACCGGCGGGCCACGGGCCCGGCTCGGCGCGGCCGCCTCCGCGGCT490730.10372658354885353No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)