FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005441522

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005441522
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences96194
Sequences flagged as poor quality0
Sequence length151
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGAAAAATCTAATGTAATGGGTCCACCAAAACATTAAATAAATAATCTAC43764.549140279019481No Hit
ATAGAACTAGGAAAAGAATCCCTTGTGCTCAAACAACAAAAGGTGGTTAT40084.166580036176892No Hit
AGTTGGACCTGTTTCTCTTGAGCTCTGGCATTATCTATGGCAACTTTCAA35463.6863006008690773No Hit
GCTCTGAACTTTAGCTACCGTTAAATTAACAAAAATACTACAATTTCAAA32653.3941825893506876No Hit
ACTTTCTAATGTTTCTGGGTCCTGAAGAATTAAGATACAAATTAATTTTA29983.116618500114352No Hit
TGGCATAAGTGAGTAGCAGTCTTACCTGTTGGTGGAATTATCCCAGGAGA26402.7444539160446597No Hit
GAGAGGGCTCTGTTTGTCGCTGGCAGGCCTGTCCAAGCCCAGCGCTTCTG19512.028193026592095No Hit
CCTGATAACCCAGGCCTGTCTGACTACTTTGCCTTCTTTTGTAGCATATA18801.9543838493045302No Hit
CGCCCCAACTCCTCTCTCAGGGCGATGAAATCACGGTGCTGCTGCAGGGC14881.5468740254069901No Hit
ATTTGTGGTTTACTTGGTTTTCCTCACTCTGGAGCAGGAGCAATTCAGTT12721.3223277959124269No Hit
AAGTGATCACAATAAGTTTCAGCGAACATAAAACTTACCGACACTTGAAT12011.2485186186248622No Hit
TTTAATATCTTCAGCTCTTTTTGTGGATATAAAATGTGCAGATGCAATTG11611.2069359835332765No Hit
CTTGCCGCCACACAGTTCCAGCAGAGCCAGGCCAGCCTCCTCCACATGCA11561.2017381541468282No Hit
GGGCACTAGCTAGGGTTCAATATACAAATAAAAGTGGCTGACACAGAGTT10681.1102563569453396No Hit
TCTTCCAGGTTGTAAAAAGGCCTACCTGTATGTTAGTCAGAAGAGTCTCG10441.0853067758903883No Hit
AGAATTAGTGCCAGAATTTTAGGAACTTCAGAGATCGTGTATTGAGATTT10351.0759506829947814No Hit
ACCCATTAATGGTTCACCTCGAACACCCAGGCGAGGTCAGAACAGGAGTG10111.0510011019398298No Hit
CGTTCGTGAATTCCACCAGATCTAACAGCTCCTTACTTTCAGGGTTGAAG9871.0260515208848786No Hit
ACAGTTATTCATGCAATAATATACACCCTCTTACCATTTAAGACCCTGAG9851.0239723891302992No Hit
ACCAACCTGTATAACTAAAACAGGGAAAAAATTAAGTTACAGACTTACAA9290.9657567000020791No Hit
AAGATACCAGATCATGTCAGAGAGAGAGCTTGGTTAACTTGGGAGAAAGT9050.9408071189471277No Hit
TGGTTTCTGGAGGACATAATTCTGTAAGTCTGGGTAACCACTGCTACAAG8370.8701166392914319No Hit
ACACATTTTCCTATTTTTATCCCCTCTAGGACTGTTATGAACACTATCCA8340.8669979416595629No Hit
TGAGACAACAGAAGCATTATACTGCTTTTTTGATGCATAAAGCACAAATT8170.849325321745639No Hit
GTATACTACTGCATACATAATACCTTCCTTGTGTGTTTTATGTTTATATA8090.8410087947273218No Hit
TTTGAGGCTAAAAGAAAGAAAATGGTATTTTTTAAGAACAAAACCATGTA7950.8264548724452668No Hit
TGTGGCAGCCTCCCGACCTTTCTCTGAGCCCAGTCCACACAAGCTAGAAT7930.8243757406906875No Hit
CAGTTGGGGTTCGGCTTTAGGTTCTTGTCGGTGGGGACATCTGGATGTAA7840.8150196477950807No Hit
GTGTTTCTTTCGGAATCTCACTTCCGGAGAGCACTAAGCCACTTCCAGCC7160.744329168139385No Hit
GCTGTTAACAGTTCTTCATCCTTTTTCCAGCTTCTACTCGAACACGAATG6830.7100234941888267No Hit
AGAAAGTAAATATTCCTTCAATGCTATGCATTTCACAAAATTTAATAAAG6720.6985882695386407No Hit
TGGGGGTCACATCCACACTTGGTGGGGCACTGCCCACACAAAATGGTTTG6600.6861134790111649No Hit
CATATGCAAAGTGAAGAATATAGACCTTAAATTCAAAATCATTGTAACAG6520.6777969519928478No Hit
TACATTTCCAAGCAACCTCAACATATTATGTTAGTTTTCAATATTTTACA6500.6757178202382685No Hit
ACGATCATACAATTTTAAGATGACAGAAAAATCATAAAATGTATAGTGAC6470.6725991226063995No Hit
ACAGTTTTAACATAGTATCCAGTGTGTGAATTATTTAATGAAATATTTGA6270.6518078050606068No Hit
CTATCCTGCCACTGCCACAGCTGCTACTGAGCGCCGACCAGCAGGCGGCC6180.642451712165No Hit
GCCGCCAGCAGCCTGACTGTCCCTCTGTCCTGCCAGCCACCCTCTGCCCC6130.6372538827785517No Hit
GCCAACTTGAAATGAAGACTTTTCCTTTAAATATATCTAGGTATCTTTCT5980.621660394619207No Hit
CATAGCCACCCTTCCCTCTGACAAGGGTGGGAAGCACAGCACATTTTAAC5930.6164625652327588No Hit
CCTCCTACGGAACTAGAGCAAACAATGCAGCTTACCACAAACAGAGCTGC5560.5779986277730419No Hit
AATTTGCAGGTAGATTGGAGCAAATTTATTGCTGTGAATGGAGCAACTGC5290.5499303490862216No Hit
GTGGATAGACGACATGGAGGTTTATTTCAGGAACCCCGGAGGCATGGCGG4900.5093872798719254No Hit
GGCCAACACAAGCAAACTGCTCAGGTTTACTTACATCTCTCACTTTCGTT4850.5041894504854773No Hit
CATCATAGTTACTGGAAATTTGAGTTTTCCATTTATAAATACACATGAAA4840.5031498846081877No Hit
ACATGTAAAGGATAATTGTCAGTGACTTTTTTCTTTCAAGGTTGAAAATC4820.5010707528536084No Hit
CTGCAAAGTTCTTTTCTATAACATGGATTTCTTCAACAGGAAAGATTCAG4640.4823585670623947No Hit
AAGTCTTGTCTTCCTCCCCTCCCAGGTGGCTACCCCAGGCAGCCAAACTA4440.4615672495166019No Hit
TCTGACCCATTCCTATGAATTTTGACCTGAACCTTCCAGAAATCCAGTTC4360.45325072249828474No Hit
CCTCGACATTGATTTCTGTTTTTACCTCCTAAAGAACTGCACAGTGAATC4300.44701332723454684No Hit
TCCACAGGAAGATCTTTGTCCTCTGGGGCTTCAAAGGGATCACGGTGCTT4270.44389462960267795No Hit
GCTCTCCCGCCAGTGTTGCTCAGTCTCGCTCAGGACCACTCTCGCCTGCT4240.44077593197080894No Hit
TTATTTTGTGCTCATTTAAGTTTCAAACTTACTATTTTGACAGTTATTTT4180.4345385367070711No Hit
ACAATTTTCATAGGTCCTACATGAAGATGAGTATGTTCTGTTCCAAGGGC4060.4220637461795954No Hit
GCTTAAGATGAACATGCCAAAATAATTGTATAAAAAAAGAATTACCTTAA4050.42102418030230576No Hit
TGCTTCTTTTCAGATATGCAAAATGTTATTTCATTGGGTTGTTGTCAACA4000.4158263509158575No Hit
GTCCTTCTGACTGGAGTAATTTCTCTTATATAAAGAAGAGATATTTTCAT3960.411668087406699No Hit
TCTAGATTTATAGCCTTCTGCAAATTCCTACCTTGTATTGTCCTTTCAGC3870.4023119945110921No Hit
CAACTGGTAAAGTAACTGAAATTCAGGTGGGGGGGCTCACCTGCAATGCC3670.3815206769652993No Hit
TCAGGTATCCAGCCCTGCTCCTGGACCAAATGCACCCTCTAGGACCCTCC3590.37320414994698214No Hit
TGCTGACACAAATAAGGTTTCAATTAAACAACTTCTTTTTTTTTTTTTAA3460.35968979354221675No Hit
GCGTCTGTGTGTCCAATACCATTCGAAGCCTGTCATTTGTGCCAGGCAAT3440.3576106617876375No Hit
GTGAACACTCAAGGGGTGGCCCCCGCATACCTTGTTCATTGTCACTGGGT3430.3565710959103478No Hit
ATCCTGTGTTTCTTTGCCTCCTATAGACATGATTCCTATGGCAATCAGTT3400.3534523982784789No Hit
CACAAACTCTGGACGGCCGGTGGGAAGCCCATAGGGCAGCCCAGGCTTTG3330.3461754371374514No Hit
AGAATCAGGGACAACAATAATGCTTTAGGAATACATGGGACACAAGGTAC3280.3409776077510032No Hit
TCTCTCTCACTTTCCATCTTCTTCCTTAGGCCGCCAGGCTACCCCAATAT3170.3295423831008171No Hit
TGTTACCAAGATTATTTTTGACCTAAGTTATAGTTAGAATACTTCATTAT3160.3285028172235274No Hit
TCAGGTATCCAGCCCTGCTCCCCTGCCCCGGCCAATGGAGAACCGCACCT3130.3253841195916585No Hit
TGTGCTTCTTACCAGTCAAAAAGTATTATAAACTATTAGAAAAGAAAATC3100.3222654219597896No Hit
AGAACGATTATCCATTCAAAATTTTAGGTAAATTTTTTACTTTTAGTAAA3000.31186976318689313No Hit
AGCTCAGGTTAATGAAATGCTCTTTATTTTGTAGCCATCCAGTCCAATGG2990.3108301973096035No Hit
AGCAAACTACTCAACTTGTATCTCTGTCCACAGCGGGATCAGGATCTATG2870.2983554067821278No Hit
GCATGAGAAAACTACTATGACTTCTAAATTACGAAAAAATGTTAAAAAGT2820.2931575773956796No Hit
GGATGCCACATTTCATAACTGTTTTTAATGGTAAAAAAAAAAAAAAAAAA2800.2910784456411003No Hit
GTTTTCAATCACATGACAGCCATGCTCACCCCTGAGCCACAGATACTTGG2700.2806827868682038No Hit
TGAACTGGCAATTGTCTATTTATCTTTTATTTTTTTAAGTCAGTATGGTC2680.27860365511362456No Hit
CAGCATTATAATTAGAGCGATTTCATGATTTGAAAAAAATCTACTTGTAA2660.27652452335904526No Hit
TGAAACACTGGCATTTAATGATTTAAAGTAAAGAATTCTGTAATTTGTAG2510.26093103519970057No Hit
TTGAATCGGGAAAAACAAGGAATATATAATCATACATTACCTTTTTCAAT2470.256772771690542No Hit
CCCTCCCCTACACCTAAAGGTGTATTTAAACTATCTTGTGTGATTAACTT2390.24845624467222488No Hit
ACCTCAGAATGTAAAAGAACTTACTGATTATTTTCTTCATCCAACTTATG2370.2463771129176456No Hit
TGAGCTACACAAAAGAATTATGTTCCAGTATTGCAAGGAAGACCTAAAAG2370.2463771129176456No Hit
AATATGCCACCTCAGGTTGGGTCAGGGATGTGTCCCCCACCAGGGGGCAT2330.24221884940848704No Hit
TCTTTCAGTGATACATTTTTCCTGTTTTTTTTCTGCTTTCTATTTGTTTA2290.23806058589932846No Hit
GGGGCAACAGGAGAGCTGTGTCCCCCGGCAGGCCACTGGTGACCGCCTTG2280.2370210200220388No Hit
TTGGAAATGAAATTTAACTCAAAAGGTTTATGTAAGATTCTAGTAATAAA2280.2370210200220388No Hit
GGAAAAATCTAATGTAATGGGTCCAACGGGCTAATATTTATCGGTGGAGC2260.23494188826745951No Hit
CAGTGGCCTGCTGCACTGCTAATGTTCTCAAGCCACAGCTTGCTTGTCTG2230.23182319063559056No Hit
TTGTTCACCGCTTGCCTTTCTACGCTCAGCTCCAGGGAGTGATCCCTTCA2160.22454622949456304No Hit
AACCCTGAGATCACCTCTTAGAACTGGTTTTAACCTTTAGCTGCAGCGGC2150.22350666361727342No Hit
GCTCATCTCTGCAAAATTGTATATGGTTTTTTATTACTAATTGGTATTTC2110.21934840010811485No Hit
CAGCCCCAGGGAGCGCCCACAGGGACACACCTCATAGCCAAGCCTGGCTG2100.21830883423082523No Hit
CTATCCTGCCACTGCCACAGGCCCATGAAGAGTGCTACCAGACTACTGCT2090.21726926835353555No Hit
CCAGGCTGAGAACAAGACACATCACTGACCTGGTTCCATCCTGGGCACTA2060.21415057072166666No Hit
TTAGGAGATTGTTGAAAAGGAAAAGAAAATTAAATACAATAACAGTAAAC2010.2089527413352184No Hit
GGCAGGCGGGCGAAGCAGACAGGGCTCACCTTTAGAGACTCGTCGGCGTT2010.2089527413352184No Hit
TGAACTCTTCTGCAAAAATGGATATTATTAGAAATTAGAAAAAAATTACT1860.19335925317587377No Hit
TGAAACAAGTATGTACTGAATCAATTTGATTTATAAGTATATGCCAATTA1820.18920098966671517No Hit
GAAGAACTTCTAGGTCCTAAACTAGAAGAGGAAGAAGAAGAGGAAGTAGT1810.18816142378942552No Hit
AGCCACTATGGATGACATGTTGTCTACTCGGTCTAGCACCTTGACCGAGG1810.18816142378942552No Hit
TGGTGACAAGTAGAAGTATGAGACCTACGGATTCTGTCAGAAGAGCTCTC1730.17984489677110838No Hit
CAATGGGGGTGGCGGCATGCAACTGTGGTTCCAGCTACTTGGGAGGCTGA1700.17672619913923945No Hit
TAAAAGCTACCACCTCATCCTGGGCACCCTGGTTATATCAACTTCAGCTA1630.16944923799821196No Hit
CAATGGGGGTGGCGGCAAGGCAGTGAGATGATAGGAGATGTGATCAAAAA1610.16737010624363266No Hit
TACTCCCAGCAGCCATCCCAGCCTCCACATCAGCAGTCCCCGGCTCCATA1600.166330540366343No Hit
AATGTGGTTTTAATTTCATCATGTTTCATATAGGATTCACCTTTATTTGA1590.16529097448905336No Hit
AGCAGGCTCTTATTTTTCTTTTTGTTTGTTTGTAGCGATACAAACTTGGA1550.1611327109798948No Hit
TTTTCCCGCGGTTGGACGAGTTGACGGGCTAATATTTATCGGTGGAGCAG1550.1611327109798948No Hit
GGCAGGCGGGCGAAGGTAGCTCACGCCTGTAATCTCAGCACTTTGGAAGG1530.15905357922531552No Hit
ACCTAGCTCAAGGGTTAATATTTCATAAATAGTTACTTTTTTTTTTCATT1500.15593488159344657No Hit
TATAGAGCTTGGTGGAAGGCCGGGTGGAGCGTGCAGTTAGCCCACGTTCC1460.151776618084288No Hit
TCCGGGAAAACACCTTGGTTACACTCGCCAACATCTCGGGGCAGTTGGAC1450.15073705220699837No Hit
TCCACTCAGGCCCGGCCAGGAACACTTTCTCTAGTCTGTAACACCAGCTA1430.14865792045241907No Hit
GGCTCAATCTGCCTCTCCAATTTTGTTTAGGACGGAGCCTGGAATAGGGC1410.1465787886978398No Hit
GGAAAATCTAATGTAATGGGTCCACCAAAACATTAAATAAATAATCTACT1310.13618312992494333No Hit
TGAATAAGACCATTTCTTGAACTTTGTCATACCTGTTAAGAACTTTTCCA1280.1330644322930744No Hit
GCCGCCAGCAGCCTGAGGTCCTGAAATAAGGTGTGGAACAGAGCCCCCTG1270.13202486641578476No Hit
TCCACTCAGGCCCGGCATTGGCCTGACAGGTTGACTGCCCAGACCCTTGC1250.1299457346612055No Hit
ACTGTAAGAACTTTGATACTTGTACATTTACAAACATTCTCAGGTCTCTG1210.12578747115204691No Hit
AGGTGACTGTGGCCGCCACACAGTTCCAGCAGAGCCAGGCCAGCCTCCTC1160.12058964176559868No Hit
TCCACTCAGGCCCGGCCTCTGGGGGATGAAGATGGTGTTAACTGCATTCA1140.1185105100110194No Hit
ACACCTGATACTGTAAACATACTGTAATAATAATGTCTCACATGGAAACA1100.11435224650186082No Hit
GGCAGGCGGGCGAAGCTTAGCCATGGTGAGTCAGTGCAGGGTTGTCTTCT1090.11331268062457119No Hit
GCCGCCAGCAGCCTGAACTTTGTCCTTGAGGGGAGATTTGATTTGCGGAA1070.1112335488699919No Hit
TGTAGAGCTGATAATCTTTTGAATTGAAATATCTATGATTTGAAAACGAA1020.10603571948354366No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)