FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005441523

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005441523
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences96194
Sequences flagged as poor quality0
Sequence length151
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGGATCAAAATAATCCCCCTCTCATTCTTTACTACTTCCCTAAAGAGAAA41764.341227103561553No Hit
TTAACATTATAGATACTCTGGCATTACGCTTCTATACCTTTTAGGTCTTC38153.9659438218599914No Hit
TAATTAGAAGCAATTAAACCTGCATGCCATAAATATTTGTCTTCCGAAAA33173.4482400149697487No Hit
CGAATATCTTCTTTGTACAGGGGCTGTTGAAAGTTGCCATAGATAATGCC30613.1821111503836No Hit
TGTAATTTTGGACTAGAAATAATGTGGCTTTGAACATGCCAGTGTTAGAC29493.06567977212716No Hit
TAACTGCAAAAGCAACCGTCCTTGAAACAAATTTATTTACAGTCCATGTT24562.5531737946233655No Hit
AAAACAAGCACACAGTATATCAGATTCCAATAGTAAAATAAAATCTAATG16681.7339958833191258No Hit
GGGTCCCGGGAAACATCGTCTATTCTAGCTTGTGTGGACTGGGCTCAGAG15201.5801401334802587No Hit
TCCGAGATTTTGGAAACTAGCAGCACCAAGTTACATCCAGATGTCCCCAC14921.5510322889161485No Hit
GAGCCATTCTCAAGATTTCCCCCCATTTCACTGGCCCTGTCTTTACGAGC12401.2890616878391583No Hit
AATAAAAACAAGAGCAAACATCACCTATATGCTACAAAAGAAGGCAAAGT12371.2859429902072894No Hit
AATGTTTGTATGTTGGTTATAGCAAGTACAGGAACTATTAAATGAAAAAT11511.19654032476038No Hit
AGCAGGAACCAATTTGCAATAAATGTTCTGGAAACCACTAGGGAAGCCAT10711.1133750545772085No Hit
AGGAATGGATGAGATACTAGGAGAGTTATCTTCTATCTATCTTCTCCTAG10351.0759506829947814No Hit
TGTTGCTTCTTATTCAAACTGTTGGTCATATGTACAGTATATAAACATAA10181.0582780630808575No Hit
GCTACTGCAAAAGAGTTAGCACTAATTACAAAAAAAACATGCTCATAACA9671.0052602033390856No Hit
AGTTAATATTAATAGTTGGGCGCACTTTTCATTTTACTGGAAACTCAAAG9350.9719940952658169No Hit
AAGCCTACAACTTACATATGTAGTAGAATCAGCCATTGCTCTGCTCCTGG8890.9241740649104933No Hit
CCTCAGCCTCCCAAAGTGGTAGGATTACAGGCATGAACCACCGTGCCCGG8220.8545231511320873No Hit
GCAAGAAAAGATTATGGATAACTACATGTTAGATAGGAGATTAGTATTTT8170.849325321745639No Hit
TCTCCATTGATTTTCACCTGTGGTTCATACCAGAGACCTGAGAATGTTTG8050.8368505312181633No Hit
AGAAACGTGAACAAATCTGAAACACTATAAAGCCATGAATAACAAAATTA7900.8212570430588185No Hit
ACTCAGTTCAGCAGGAAAACAAAATGAATAATATAAAAAATATAGACCAT7830.8139800819177913No Hit
ACCTACCTCCTGAACAGCATGAGGAAGATCCTTGTATGCTGTTACAATGA7790.8098218184086325No Hit
CCCCGCCGGAAGCCTGTCTTTAACCCTTTGTGCTTGGATGTTAAGGCTGA7580.78799093498555No Hit
CACCTTGCCCCAAAGCCTGGGCTGCCCTATGGGCTTCCCACCGGCCGTCC7300.75888309042144No Hit
CAATGGCAAGTATCCCAAGGCTCCGGAAGGGGGCGAAGGTGCCCAGCCAG7160.744329168139385No Hit
TTTCATCTGTTCGGTTCACGCCATGATAGGCGGGGCCCTGGGGGGCAGAG6930.7204191529617232No Hit
TTTCACATACTTTCCAAAAGACCAACAAATGGATGTTGACAACAACCCAA6750.7017069671705096No Hit
TGGAAATTTTTCTATGGTAGCCTAGTTATTTGAGCCTGGTTTCAATGTGA6530.6788365178701374No Hit
CCAATACCATGTAGCGCTGAGTGATAAAGTTAAAATGTGCTGTGCTTCCC6530.6788365178701374No Hit
ACAATGACTAACATTTCAAGTGGCTTAGGAATCACCCAAACAATTGCATC6460.6715595567291099No Hit
AGAGGAAAATCCAGAATTCGTTTCCTTTTATGGCAGAGGCTTATATTTAT6070.6310164875148138No Hit
TGTGAATTTACATAATAAGGTCAGACAGAATATATGATCTCATTAATGAA5990.6226999604964967No Hit
TAACTTTGTTTTGTTTACAGAGTACCTGCTCGGGCCAGGTAAATGCTATT5960.6195812628646278No Hit
ATGAATGAGATCTTAAGCATCTGGTGACATCAGAACTCTGTGTCAGCCAC5930.6164625652327588No Hit
AAGTGACTTGCCCAGTAATGTACCTAGAAAGTAGAAGAAACATGATTTGA5790.6019086429507038No Hit
ACACGCACACACACACGGGGGGGGGAGGTGTCCCACGGCTGTCATGACTG5620.5842360230367798No Hit
AAGAGGAGAGAGAGCTGGGTGTAGAGGTGTCCAGAGCACCCTCTCTCTAC5270.5478512173316423No Hit
CTCAGTTGAGCCGGCCCTGCAGCAGCACCGTGATTTCATCGCCCTGAGAG5180.5384951244360355No Hit
TTGCTGAGAAAGAGGAAGACAGGAAATGACCTTCCAGCCTGTCATGAATG4890.5083477139946359No Hit
TGCTATATGTGGCCATTACAACCTCAAGAGCGCACGCCAATAAAGACATA4800.49899162109902906No Hit
ACACGCACACACACACGGCAGTGAAAATGTCACAGAGGAAATAAAATCAA4790.49795205522173935No Hit
ACACGCACACACACACGGGGGGGGGGAGGTGTCCCACGGCTGTCATGACT4680.4865168305715533No Hit
AGAAATGCTGTATTCTTTTCAGTCACATAAACTCAGTGTAATTATGAAAT4500.4678046447803398No Hit
ATGATATCTAAAGGTCACTAAGCTAAAGACTATATAATCTACCTATATCA4440.4615672495166019No Hit
ATATTTTAAATGCATAAACAAACCTGCCAACTGAAGAAATTATACATTCT4360.45325072249828474No Hit
TGGAAACCACAGGGGCAGTCAAGTGTCAGGCGCCCACTCACCTGAGTCAA4310.4480528931118365No Hit
AGCAATAAAGCAATAATATCTGAAGCATTATTTAAGAAATCTCAATACAC4270.44389462960267795No Hit
AGGAAGTTAATTCAGAGCTAGAAATGGCTCATGAAGAGGCCAAGTACCTC4210.43765723433894005No Hit
ACTCTGCTTTACTTGCCACTCGTCTTCAATGTACCCTCCCTCTGGAAAGT4160.4324594049524918No Hit
TGTATCTGAGCACATCAAGAGACTCTTGTAGCAGTGGTTACCCAGACTTA4050.42102418030230576No Hit
AGCAAAGTACTGGCATGGAAGATATCTACAAGAGAGAAAGAGAAAGACCA3970.4127076532839886No Hit
TGTCCTCCAGAGCAATGATGATATATGTGCATTTTTTATAGGACTGGAAC3940.40958895565211967No Hit
TCTTGGGTTTTCCGGTTCATGCCCCCTGGTGGGGGACACATCCCTGACCC3880.4033515603883818No Hit
ACACAGGCAGCAGGGATATAGTATCTGACAGTATTTTATACTACTAGACA3880.4033515603883818No Hit
CAATGTTGCATTGCTTACTGTTCAAATCTTCCGAAAATTCTTACGGTTAC3730.38775807222903713No Hit
TCAGCTGTATGTTAAGAGAGACTCTTACTAACATGTTGTAAATATTACAA3650.37944154521072No Hit
CCAAAGCCAGCAACCCAGAACCGAGTTTCAAAGCAAACGAGAACGGCCTT3360.34929413476932036No Hit
AAACCCAAGAGGATCCCAAACCATTTTGTGTGGGCAGTGCCCCACCAAGT3330.3461754371374514No Hit
GGTGTGCATTGAATTAACAGGGCTCCATCCTAAAAAACAACGCCACTTGC3250.33785891011913427No Hit
ATTCTTGTATCATTTTCTAGTTGTTTTGCTATCCGTGCACTCCTGTTCTG3130.3253841195916585No Hit
TAACAAAGGACACGCAGGAGTCACTGGCTGGGAAGGCAGAGGCAAGGACC3050.3170675925733414No Hit
ATGCTCTTATTTAAAGAAGACTTTGAGCTATTTTTTTCTGTACCCTGTAA3040.31602802669605173No Hit
GAGGGAGGCGGCTTGGAGACCCTTCAGAGGGCGTTGGGTAGCCAGTGGGC3020.31394889494147243No Hit
AGAAGAGATTGAGGACGTGGCTCTTCAGGCAGGACTGGCCAGTCAGGTGA2970.3087510655550242No Hit
GCTTGCTGCTGACCCAGCGTTTTCTGTTTCCATGTGAGACATTATTATTA2960.3077114996777346No Hit
GATGGAAAGGGGCAGATTAGGCAACCGAATGAGGAAGATCTCCTTACCAA2940.3056323679231553No Hit
CCTCAGCCTCCCAAAGTGGTAGGATTACAGGCGTGAGCCACTGCGCCCGG2930.30459280204586564No Hit
CTCGGGGTATGGAGATAGGTCCAACTGCCCCGAGATGTTGGCGAGTGTAA2840.29523670915025885No Hit
CATCATCTCTGGGCTGGCTGCCATCCCTGTAAAAGAGAAAAAAATTCTCA2780.28899931388652095No Hit
AAGGCAAATGAAATAGCTTTTATGGTAAGGTGTTGTTTTTTTTTTTTTCC2780.28899931388652095No Hit
TTTGGACAGCAACAAGGGTCAAGGTAATCACAATCACCATCTACCTGCTG2690.2796432209909142No Hit
TGGACCCATTACATTAGATTTTTCCAATCAAAGGATACTTTTGACCTACC2670.2775640892363349No Hit
CACCAATGGGGAGCGCTTTCTCTACCTGCCGCCACCTCACTACGTCGGTC2660.27652452335904526No Hit
ACACAGGACTTAAATCTATGGGCTTAATTTATGAAGTAGCCTGCTATAAT2660.27652452335904526No Hit
CCTCAGCCTCCCAAAGTGGTAGGATTACAGGCGTGAGCCACCGCGCCCGG2650.2754849574817556No Hit
ACTCACTTGGGAAAGAATCTTGTTATTTAAACTCCTTGTTGTTGTTGACA2610.27132669397259707No Hit
TCAGTTAACAAGTAAGTAGGGAGGAGAGAAGGTGAAGTGCTTGATTTTCT2500.259891469322411No Hit
AGTTACTGTTGAGTCCAAGAGAGTTTAATTGACTTATTGGAACCTTGTGT2450.2546936399359627No Hit
AAAGCCAAATTGTTTTGCAATGTTAAGAAAAAAATTGGTTTATGCTTATA2370.2463771129176456No Hit
CCAAGGTTGTTTCTAGTACCAGAATTATAGGAACTTGCTAACAGTTAATA2320.24117928353119736No Hit
TGTATGATATTCATGCAGAAACAATGATGAAATGGAGTCAAGTTTTCTAG2310.2401397176539077No Hit
AGCTACCTTAAATATCCCCTATTTTAAGTTACAGCATCTCTAAATAAGTT2310.2401397176539077No Hit
GCATTCCTTCTCCTTAACCTCACACTATCCCTCTCCCCCGACCAAAGAAA2240.23286275651288021No Hit
AGGCTCCTATAATTAACAGTCTGTTTATGGAGTAAAATTAATTTGTATCT2240.23286275651288021No Hit
CCGGGACTGAGGGGAGCCCACTGCCCTGAAGGGTAAGTCCCTGATCAGCC2200.22870449300372164No Hit
TGCATATCTGGAATTACACCTTTAAAGACTGATATGAATCAGTACGGTCA2180.22662536124914237No Hit
TGGACCCATTACATTAGATTTTTCCAGATCGGAAGAGCGTCGTGTAGGGA2160.22454622949456304Illumina Single End PCR Primer 1 (100% over 25bp)
CTCCAAAGACCTGGAAGCCTTCAACCCTGAAAGTAAGGAGCTGTTAGATC2110.21934840010811485No Hit
ACATATGATGTTTAGTCACGTGAAATTTCAGACCCATCTAAGGTATGTAA2110.21934840010811485No Hit
GGCATGAAAGAAATTGGTATAAGCCAGGAGCAGTGCTGAGACTCTGGATT2070.21519013659895628No Hit
TCACCAATTAGGAGTATGATTTGAGATTAAACTTGATTTGAAAGTAAATC2050.21311100484437698No Hit
GATAGGTGACTGTCCGGAGATTCAGAAAGCGGCCGGAGCCCAGTTCGGAC2010.2089527413352184No Hit
ATAAACTATAAGTTGATTGGCTGCTGAGACAGCAAGGACTTTTTACAGAG2000.20791317545792876No Hit
GGCCTGAGGGTGTATGTCTTTGAAAGCTACAGAGAGGTCCACCTTACAAG1950.20271534607148056No Hit
TAACCACATTTGCGCTCTATGCAAGCCCTTGGAACAGAACATACTCATCT1900.19751751668503234No Hit
TCCTTACCAATACTCCATCCACAGATGAAACTTTCTCCCAAGTTAACCAA1870.1943988190531634No Hit
TTGCTCTCGAAGTGGGTCTGGATATGCTCAGTGGTGTCCCCCCCACCAAT1780.1850427261575566No Hit
CCTCAGCCTCCCAAAGTGGTAGAATTACAGGCATGTAATTCTATGTAATT1780.1850427261575566No Hit
TGCTGATGAAGTAAAGTGTTTATTCTCTTTACATTTCTTATTGTATTGAG1770.18400316028026695No Hit
AGGCGAGTATAGTGTTGGAAACAAGCACCGTGATCCCTTTGAAGCCCCAG1710.1777657650165291No Hit
CCTGGGTGTTTGGACATCTCAAAGTCATTGCCTGGCACAAATGACAGGCT1660.17256793563008088No Hit
CAACTCGTCCAACCGCGGGAAAAAGATCGGAAGAGCGTCGTGTAGGGAAA1660.17256793563008088Illumina Single End PCR Primer 1 (100% over 27bp)
TTTCCCTCAGGCCCTATTCCAGGCTCCGTCCTAAACAAAATTGGAGAGGC1590.16529097448905336No Hit
CATATGCACATGAATGAATTTCTTCAATTATTTAGCTACTTACTGAGAGC1580.1642514086117637No Hit
ACACAGGCTTGAGGCCAACCATCAGAGCTTAAACTGGGAAGCTGGTGGTA1550.1611327109798948No Hit
ACACGCACACACACACGGAAAATTTTGCAAAGGTTAGACATTTTTAACAC1530.15905357922531552No Hit
AAACCAAGAGTACTATCAATAATCCTCATTTAAAAACATAAGTTGGATGA1510.15697444747073622No Hit
TTCAAAATTCTATTCCCTACAGTTTCTTTATAGAATGTTACATTGCACTT1480.1538557498388673No Hit
AGCTGGGACTACAGGTCTACACCACCACGCCCAGCTAATTTTTGTACTTT1430.14865792045241907No Hit
TGTAGTCACACACAGGGAAGGGCAGGAGGCAGGGATATCTTACCTGCGGT1390.14449965694326047No Hit
CCCCGCCGGAAGCCTTCAACCCTGAAAGTAAGGAGCTGTTAGATCTGGTG1330.13826226167952263No Hit
TGCCTTGGGTGGAGAACTGATTGCCATAGGAATCATGTCTATAGGAGGCA1310.13618312992494333No Hit
CCTCAGCCTCCCAAAGTGGTAGGGTTACAGGCGTGAGCCACCACGCCCAG1220.12682703702933656No Hit
CAGTACCCAAGGTGCACATGTGCCCAGCTTTGCCTGTTGCTTTTAAAGGA1210.12578747115204691No Hit
CACCATAGTGCTATACAACTTCTCCAGGCGGCTGAAGGGGGGTGTGGCCA1190.12370833939746761No Hit
GCTTGCTGCTGACCCAGTGCTTGAAGCATAGTAAGTGCTCAGTAAGAATG1180.12266877352017798No Hit
ACACGCACACACACACGGATAGAGATCACTGTCTGCTCATATTACTTATC1150.11955007588830904No Hit
ATAGTGTTAATCCTTGTGCATATGTGTCATAATACAACTATTTCTGTAAA1130.11747094413372976No Hit
TCATTTCTAGATGGAAGACTGTATTTGAAAACTACTGTCATGTACTGAAT1080.11227311474728152No Hit
GACCCCCGGCGTCCTGCTCACCCGGCCAGTCATCCTGGCTATGGACCACT1060.11019398299270224No Hit
ACACGCACACACACACGGTATCTTGATGTTTTAATTATCTTACTAATAAA1000.10395658772896438No Hit
CCCAGAATCTAATTGTGAACAATGACATAAAAAATCAGAGTGTAACCCTA970.10083789009709546No Hit
CCTCAGCCTCCCAAAGTGGTAGGATTACAGGCGTGAGCCACCACACCCGG970.10083789009709546No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGTCAGT100.0070898593144.93916145
GCCTTTA100.0070898593144.939169
GGAGCCG100.0070898593144.939169
TTAATCC100.0070898593144.939167
ATAGGTG202.1251562E-6144.939162
GTTTCGG100.0070898593144.93916145
GACACTG100.0070898593144.93916145
GGACTGA550.0144.939164
TAGAAGC3450.0144.939165
TCGGGGT351.0913936E-11144.939162
GAATATC3300.0144.939162
GCTGACC550.0144.939168
TCTAGAT100.0070898593144.939166
GCGCACC100.0070898593144.939169
TTGAGGA400.0144.939169
CGGGGTA351.0913936E-11144.939163
CTGCGAA351.0913936E-11144.93916145
GTTAACA400.0144.939164
GTTCGGT750.0144.939169
TACTGGC650.0144.939168
Produced by FastQC (version 0.11.5)