FastQCFastQC Report
Fri 27 May 2022
EGAF00005441984

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005441984
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences254323149
Sequences flagged as poor quality0
Sequence length98
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT50685721.9929652569691956Clontech SMART CDS Primer II A (100% over 26bp)
GCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT12122210.476645954081042Clontech SMART CDS Primer II A (100% over 24bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG6989790.27483892156431267No Hit
AGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT4612920.18138026436594648Clontech SMART CDS Primer II A (100% over 25bp)
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2747980.1080507225081583Clontech SMART CDS Primer II A (100% over 21bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)