FastQCFastQC Report
Fri 27 May 2022
EGAF00005442245

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005442245
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences312826291
Sequences flagged as poor quality0
Sequence length98
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT73286192.342711981327682Clontech SMART CDS Primer II A (100% over 26bp)
GCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT15895820.5081356796830098Clontech SMART CDS Primer II A (100% over 24bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG8503110.27181570873785665No Hit
AGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT6273760.20055091852877543Clontech SMART CDS Primer II A (100% over 25bp)
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3643340.11646527497268444Clontech SMART CDS Primer II A (100% over 21bp)
AGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3322720.1062161364180225Clontech SMART CDS Primer II A (100% over 22bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)