FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005443764

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005443764
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences25280858
Sequences flagged as poor quality0
Sequence length150
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATTAT51400.022.159241
GTATAAG57550.021.5448781
TCTGTCG84450.021.4844268
GTATTAG55000.020.7088181
GTATTAA50000.020.328721
GTCTTAC88100.019.9652561
GTCTCGC97900.019.8811741
GTGTAAC75550.019.846771
GTCTTGC210400.019.5637131
GTGTTAG116900.019.4864851
GTATTAC49650.019.3104921
GTGTAAA100250.018.6241441
GTGTAAT75550.018.510931
GTATAAT48800.018.4650761
GTATAAC35950.018.4480251
GTATATA61400.018.3154321
GTATAGG59200.018.0219171
GTATAAA72350.017.635821
GTGTATA84600.017.553261
TAATACT50050.017.550064
Produced by FastQC (version 0.11.5)