FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005444600

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005444600
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1602506
Sequences flagged as poor quality0
Sequence length301
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAATCTTACCCCGCCTGTTTACCAAAAACATCACCTCTAGCATCACCAGT595983.7190500378781737No Hit
GCCATACTAGTCTTTGCCGCCTGCGAAGCAGCGGTGGGCCTAGCCCTACT595883.718426015253609No Hit
AATTAGGCTGTGGGTGGTTGTGTTGATTCAAATTATGTGTTTTTTGGAAA426782.663203757115418No Hit
GGCAGGTCAATTTCACTGGTTAAAAGTAAGAGACAGCTGAACCCTCGTGG370952.3148119258211826No Hit
AATTAGGCTGTGGGTGGTTGGCCATGGGTATGTTGTTAAGAAGAGGAATT159060.9925703866319376No Hit
GGCAGGTCAATTTCACTGGTAGTAATATAATTGTTGGGACGATTAGTTTT119180.7437101639557043No Hit
AATTAGGCTGTGGGTGGTTGTATATAGCCTAGAATTTTTCGTTCGGTAAG65910.4112933118503145No Hit
AATTAGGCTGTGGGTGGTTGTGGGTATAATACTAAGTTGAGATGATATCA30290.18901645298051928No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGTCTT80700.0229.919898
AGGCTGT96550.0212.340065
ATCTTAC86150.0205.958533
AGTCTTT92600.0200.532219
ATTAGGC103650.0199.21782
CTTACCC89850.0196.984745
GCCATAC97050.0191.945191
ACCCCGC93100.0190.266688
TACCCCG96900.0183.109667
TCTTACC97500.0181.5294
TTAGGCT114500.0180.468783
CCCCGCC98950.0179.167029
GCTGTGG115650.0176.25117
AAATCTT102750.0172.540911
AAGGTCC351.0069598E-6168.56265295
TTACCCC106600.0166.586076
GCAGGTC96600.0162.455322
CAGGTCA98400.0159.183793
GTCAATT101450.0155.1256
TAGGCTG135700.0152.057314
Produced by FastQC (version 0.11.5)