FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005444601

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005444601
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1602506
Sequences flagged as poor quality0
Sequence length301
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAATCTTACCCCGCCTGTTTACCAAAAACATCACCTCTAGCATCACCAGT585973.6565853731592894No Hit
GCCATACTAGTCTTTGCCGCCTGCGAAGCAGCGGTGGGCCTAGCCCTACT576573.597927246450247No Hit
AATTAGGCTGTGGGTGGTTGTGTTGATTCAAATTATGTGTTTTTTGGAAA454592.8367444490067433No Hit
GGCAGGTCAATTTCACTGGTTAAAAGTAAGAGACAGCTGAACCCTCGTGG387782.41983493353535No Hit
AATTAGGCTGTGGGTGGTTGGCCATGGGTATGTTGTTAAGAAGAGGAATT148860.9289200789263815No Hit
GGCAGGTCAATTTCACTGGTAGTAATATAATTGTTGGGACGATTAGTTTT94180.5877045078146353No Hit
AATTAGGCTGTGGGTGGTTGTATATAGCCTAGAATTTTTCGTTCGGTAAG66030.4120421389997916No Hit
GGCAGGTCAATTTCAGTGGTAGTAATATAATTGTTGGGACGATTAGTTTT27150.169422142569201No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGTCTT75600.0223.824368
ATCTTAC87400.0216.021263
AGGCTGT105050.0215.27065
ATTAGGC110750.0204.032532
CTTACCC93050.0201.972245
AGTCTTT86800.0194.77999
TTAGGCT117100.0193.100423
TCTTACC97900.0192.864524
ACCCCGC97150.0192.397558
GCCATAC90000.0188.275651
GCTGTGG121400.0184.831627
TACCCCG101300.0184.661157
AAATCTT106700.0176.913991
CCCCGCC107200.0174.228129
TTACCCC109750.0170.707026
TAGGCTG136050.0166.973374
GCAGGTC95950.0165.406162
CAGGTCA98750.0161.019913
GTCAATT99050.0160.701226
GGCTGTG139950.0160.433096
Produced by FastQC (version 0.11.5)