FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005444602

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005444602
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences384436
Sequences flagged as poor quality0
Sequence length301
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCATACTAGTCTTTGCCGCCTGCGAAGCAGCGGTGGGCCTAGCCCTACT92402.403521002195424No Hit
GGCAGGTCAATTTCACTGGTTAAAAGTAAGAGACAGCTGAACCCTCGTGG73751.9183947393064127No Hit
AATTAGGCTGTGGGTGGTTGTGTTGATTCAAATTATGTGTTTTTTGGAAA65121.693910039642489No Hit
AAATCTTACCCCGCCTGTTTACCAAAAACATCACCTCTAGCATCACCAGT51411.3372837091219345No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGTCTT13400.0228.955238
GGCGGTC304.666217E-7196.666672
GCAGGTC13000.0191.752
CAGGTCA14250.0172.859663
GCGGTCG351.0057138E-6168.571433
GCCATAC18400.0168.342391
AGTCTTT18400.0168.342399
GTCAATT14750.0164.06
AATTTCA16050.0150.71659
ATTAGGC16000.0146.578122
GGCAGGT18500.0136.337831
TCTCGTT651.0059011E-9136.15385295
AGGCTGT17150.0135.88925
GGTGCCA352.1219596E-4126.4285661
GTCCATG352.1219596E-4126.4285664
AGGTCAA19550.0125.2429664
TCAATTT19750.0123.2278447
CCATACT25550.0122.3874742
GGTCAAT20850.0117.434055
CTAGTCT27700.0111.290627
Produced by FastQC (version 0.11.5)