FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005444604

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005444604
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1121817
Sequences flagged as poor quality0
Sequence length301
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAATCTTACCCCGCCTGTTTACCAAAAACATCACCTCTAGCATCACCAGT254242.2663232951542005No Hit
GCCATACTAGTCTTTGCCGCCTGCGAAGCAGCGGTGGGCCTAGCCCTACT193331.7233648625399687No Hit
GGCAGGTCAATTTCACTGGTTAAAAGTAAGAGACAGCTGAACCCTCGTGG191631.7082108757488965No Hit
AATTAGGCTGTGGGTGGTTGTGTTGATTCAAATTATGTGTTTTTTGGAAA155961.390244576432698No Hit
AATTAGGCTGTGGGTGGTTGTATATAGCCTAGAATTTTTCGTTCGGTAAG63680.5676505169738023No Hit
AATTAGGCTGTGGGTGGTTGGCCATGGGTATGTTGTTAAGAAGAGGAATT31180.27794194596801436No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGTCTT31450.0205.876688
AGGCTGT50000.0169.908775
ATCTTAC46650.0168.831273
ATTAGGC53400.0159.366792
CTTACCC50050.0157.362215
AGTCTTT41250.0156.60789
TACCCCG51400.0152.368337
ACCCCGC54000.0144.485818
CCCCGCC55650.0140.466899
TCTTACC56450.0139.78264
TTAGGCT61200.0139.055343
GCCATAC46300.0138.88931
GCTGTGG62750.0135.62057
GCAGGTC45850.0134.46222
CAGGTCA46950.0131.6263
TTACCCC61100.0128.903096
AAATCTT61850.0128.055371
GTCAATT47950.0127.650576
TAGGCTG76350.0111.6564
CTGTGGG78350.0109.182318
Produced by FastQC (version 0.11.5)