FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005444605

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005444605
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1121817
Sequences flagged as poor quality0
Sequence length301
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAATCTTACCCCGCCTGTTTACCAAAAACATCACCTCTAGCATCACCAGT249212.221485322472382No Hit
GGCAGGTCAATTTCACTGGTTAAAAGTAAGAGACAGCTGAACCCTCGTGG192201.7132919183788442No Hit
GCCATACTAGTCTTTGCCGCCTGCGAAGCAGCGGTGGGCCTAGCCCTACT182431.626201064879566No Hit
AATTAGGCTGTGGGTGGTTGTGTTGATTCAAATTATGTGTTTTTTGGAAA158331.4113710168414277No Hit
AATTAGGCTGTGGGTGGTTGTATATAGCCTAGAATTTTTCGTTCGGTAAG60760.541621316132667No Hit
AATTAGGCTGTGGGTGGTTGGCCATGGGTATGTTGTTAAGAAGAGGAATT30050.26786900180689005No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGTCTT30950.0192.547278
ATCTTAC42900.0173.632643
AGGCTGT52550.0172.062035
ATTAGGC54750.0166.757142
CTTACCC48450.0154.047275
TTAGGCT61350.0149.545413
AGTCTTT40200.0147.875299
TCTTACC50800.0146.63074
GCAGGTC45150.0146.351842
TACCCCG50900.0144.900167
ACCCCGC51950.0142.539378
CAGGTCA47200.0140.626713
GCTGTGG65300.0138.246727
CCCCGCC54350.0135.973669
GTCAATT48500.0135.342716
GCCATAC44700.0132.634871
AAGGTCC1250.0129.73793295
TTACCCC58100.0128.212956
AAATCTT59150.0126.163841
CTGTGGG75500.0119.960448
Produced by FastQC (version 0.11.5)