FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005444606

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005444606
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1264404
Sequences flagged as poor quality0
Sequence length301
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCATACTAGTCTTTGCCGCCTGCGAAGCAGCGGTGGGCCTAGCCCTACT277512.1947890073109546No Hit
AATTAGGCTGTGGGTGGTTGTGTTGATTCAAATTATGTGTTTTTTGGAAA240351.9008955998241066No Hit
GGCAGGTCAATTTCACTGGTTAAAAGTAAGAGACAGCTGAACCCTCGTGG221721.7535534528520949No Hit
AAATCTTACCCCGCCTGTTTACCAAAAACATCACCTCTAGCATCACCAGT220581.7445373472402808No Hit
AATTAGGCTGTGGGTGGTTGTATATAGCCTAGAATTTTTCGTTCGGTAAG30170.23861044413019888No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGTCTT38300.0205.250898
AGGCTGT56000.0170.401285
GCAGGTC42700.0168.212272
ATTAGGC60250.0158.626052
CAGGTCA45950.0156.956733
AGTCTTT50550.0155.511559
GTCAATT47850.0149.183216
GCCATAC54900.0144.801481
ATCTTAC49000.0140.565053
TTAGGCT69250.0138.223373
GCTGTGG71400.0133.648067
CTTACCC51750.0133.095415
AATTTCA54150.0132.91629
TCTCGTT2150.0130.3382295
ACCCCGC53900.0126.691888
TCTTACC55700.0124.186454
TACCCCG57200.0119.382747
CTGTGGG82150.0116.3386468
GGCAGGT62650.0115.3537141
CCCCGCC60000.0113.811559
Produced by FastQC (version 0.11.5)