FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005444607

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005444607
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1264404
Sequences flagged as poor quality0
Sequence length301
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCATACTAGTCTTTGCCGCCTGCGAAGCAGCGGTGGGCCTAGCCCTACT268292.121869275959266No Hit
AATTAGGCTGTGGGTGGTTGTGTTGATTCAAATTATGTGTTTTTTGGAAA247911.9606866159866625No Hit
GGCAGGTCAATTTCACTGGTTAAAAGTAAGAGACAGCTGAACCCTCGTGG228111.8040910974656834No Hit
AAATCTTACCCCGCCTGTTTACCAAAAACATCACCTCTAGCATCACCAGT212451.6802382782718184No Hit
AATTAGGCTGTGGGTGGTTGTATATAGCCTAGAATTTTTCGTTCGGTAAG27900.22065732155228868No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGTCTT42900.0195.34978
GCAGGTC46200.0170.823472
AGGCTGT54750.0165.990755
CAGGTCA47900.0164.767363
ATTAGGC56750.0160.121862
GTCAATT50100.0157.550776
AGTCTTT54100.0154.907629
GCCATAC56350.0150.775221
AAGGTCC1000.0147.42152295
TTAGGCT62450.0146.221483
ATCTTAC44550.0145.699463
AATTTCA57600.0136.529489
GCTGTGG68950.0131.6027
CTTACCC49950.0129.66315
ACCCCGC51050.0126.879218
GGCAGGT63600.0124.078931
TCTTACC52200.0123.791534
AGGTCAA65350.0120.328624
GGTCAAT66050.0119.500115
TACCCCG55850.0115.446287
Produced by FastQC (version 0.11.5)