FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005444608

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005444608
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1556375
Sequences flagged as poor quality0
Sequence length301
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCATACTAGTCTTTGCCGCCTGCGAAGCAGCGGTGGGCCTAGCCCTACT351142.2561400690707574No Hit
GGCAGGTCAATTTCACTGGTTAAAAGTAAGAGACAGCTGAACCCTCGTGG313622.015067062886515No Hit
AATTAGGCTGTGGGTGGTTGTGTTGATTCAAATTATGTGTTTTTTGGAAA238291.5310577463657538No Hit
AAATCTTACCCCGCCTGTTTACCAAAAACATCACCTCTAGCATCACCAGT228071.4653923379648222No Hit
AATTAGGCTGTGGGTGGTTGTATATAGCCTAGAATTTTTCGTTCGGTAAG22760.1462372500200787No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGTCTT48200.0228.283378
GCAGGTC49150.0193.561372
AGTCTTT65250.0168.858379
CAGGTCA57000.0166.904253
GCCATAC69400.0157.910811
GTCAATT60000.0155.363286
ATTAGGC70750.0151.770522
AGGCTGT75050.0142.681765
AATTTCA67050.0139.247519
GGCAGGT70750.0136.551771
ACCCCGC59550.0122.852068
ATCTTAC61250.0119.9239043
TTAGGCT91650.0117.482423
TCTTACC62800.0116.9644
GGTCAAT80100.0116.745265
TCAATTT80650.0115.766217
AGGTCAA81400.0115.061974
GCGGTCG651.6042395E-7113.459053
CTAGTCT97400.0112.969817
CTTACCC65100.0112.605065
Produced by FastQC (version 0.11.5)