FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005444610

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005444610
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1245536
Sequences flagged as poor quality0
Sequence length301
%GC44

[WARN]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAATCTTACCCCGCCTGTTTACCAAAAACATCACCTCTAGCATCACCAGT308422.4762030162114943No Hit
GGCAGGTCAATTTCACTGGTTAAAAGTAAGAGACAGCTGAACCCTCGTGG207321.664504277676438No Hit
GCCATACTAGTCTTTGCCGCCTGCGAAGCAGCGGTGGGCCTAGCCCTACT189371.5203896153944967No Hit
AATTAGGCTGTGGGTGGTTGTGTTGATTCAAATTATGTGTTTTTTGGAAA145051.1645588726459934No Hit
AATTAGGCTGTGGGTGGTTGTATATAGCCTAGAATTTTTCGTTCGGTAAG83010.666460062174036No Hit
AATTAGGCTGTGGGTGGTTGGCCATGGGTATGTTGTTAAGAAGAGGAATT56820.4561891426662899No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)