FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005444611

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005444611
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1245536
Sequences flagged as poor quality0
Sequence length301
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAATCTTACCCCGCCTGTTTACCAAAAACATCACCTCTAGCATCACCAGT270122.168704878863397No Hit
GGCAGGTCAATTTCACTGGTTAAAAGTAAGAGACAGCTGAACCCTCGTGG180671.4505401690517177No Hit
GCCATACTAGTCTTTGCCGCCTGCGAAGCAGCGGTGGGCCTAGCCCTACT165321.3273000539526758No Hit
AATTAGGCTGTGGGTGGTTGTGTTGATTCAAATTATGTGTTTTTTGGAAA123310.9900155435089794No Hit
AATTAGGCTGTGGGTGGTTGTATATAGCCTAGAATTTTTCGTTCGGTAAG65500.5258780155692008No Hit
AATTAGGCTGTGGGTGGTTGGCCATGGGTATGTTGTTAAGAAGAGGAATT42270.3393719651619865No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATTAGGC55300.0168.022982
TAGTCTT36150.0166.076868
ATCTTAC55850.0165.846823
AGGCTGT56200.0160.102865
ACCCCGC59300.0154.973118
TACCCCG60150.0153.028377
TTAGGCT60700.0151.137543
TCTTACC62050.0149.51924
CTTACCC61850.0148.10085
GCAGGTC50250.0144.698522
AAATCTT67000.0137.355361
GCTGTGG66400.0133.736957
CCCCGCC68850.0133.691459
GCCATAC44650.0131.461151
AGTCTTT45600.0131.336129
GTCAATT56700.0130.86036
CAGGTCA57050.0130.300343
TTACCCC73250.0125.862226
AATTTCA63400.0118.194559
TAGGCTG79050.0116.431594
Produced by FastQC (version 0.11.5)