FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005444612

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005444612
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences570433
Sequences flagged as poor quality0
Sequence length251
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAATCTTACCCCGCCTGTTTACCAAAAACATCACCTCTAGCATCACCAGT77911.3658045730173394No Hit
AATTAGGCTGTGGGTGGTTGTGTTGATTCAAATTATGTGTTTTTTGGAAA67251.1789289890311396No Hit
GGCAGGTCAATTTCACTGGTTAAAAGTAAGAGACAGCTGAACCCTCGTGG66611.1677094417749323No Hit
GCCATACTAGTCTTTGCCGCCTGCGAAGCAGCGGTGGGCCTAGCCCTACT66101.1587688650551424No Hit
AATTAGGCTGTGGGTAGAAGTAGAGGTTAAGGAGGGTGATGGTGGCTATG12790.22421563969826427No Hit
AATTAGGCTGTGGGTGTGATAGGTGGCACGGAGAATTTTGGATTCTCAGG8010.1404196461284673No Hit
AATTAGGCTGTGGGTGGGGATGATGAGGCTATTGTTTTTTGTGAATTCTT7210.12639521205820842No Hit
AATTAGGCTGTGGGTGGTGATTAGTCGGTTGTTGATGAGATATTTGGAGG5720.10027470360235119No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTACC351.2063538E-8175.04
AGGCTGT23850.0142.274635
CGTACCC455.4114935E-8136.111115
ATCTTAC17000.0128.98533
ATTAGGC28450.0124.437612
TTAGGCT28750.0121.434783
CTTACCC18150.0118.787895
GTCAATT13900.0115.449646
TAGTCTT13700.0115.346728
GCAGGTC14600.0111.592472
GCTGTGG29600.0110.912167
CAGGTCA14900.0110.167793
TAGGCTG34650.0100.4040454
GGGTTAT14250.098.85966245
TCTTACC21750.098.563224
TACCCCG22700.097.136567
ACCCCGC22900.096.2882168
AGTCTTT17300.092.760129
CTGTGGG34600.091.343938
GGCTGTG37650.089.150066
Produced by FastQC (version 0.11.5)