FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005444613

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005444613
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences570433
Sequences flagged as poor quality0
Sequence length251
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCAGGTCAATTTCACTGGTTAAAAGTAAGAGACAGCTGAACCCTCGTGG74711.3097068367363038No Hit
AAATCTTACCCCGCCTGTTTACCAAAAACATCACCTCTAGCATCACCAGT73071.2809567468922731No Hit
AATTAGGCTGTGGGTGGTTGTGTTGATTCAAATTATGTGTTTTTTGGAAA71081.246070967142504No Hit
GCCATACTAGTCTTTGCCGCCTGCGAAGCAGCGGTGGGCCTAGCCCTACT63821.1187992279549044No Hit
AATTAGGCTGTGGGTAGAAGTAGAGGTTAAGGAGGGTGATGGTGGCTATG8600.15076266625528326No Hit
AATTAGGCTGTGGGTGTGATAGGTGGCACGGAGAATTTTGGATTCTCAGG8430.14778247401535324No Hit
AATTAGGCTGTAGGTAGAAGTAGAGGTTAAGGAGGGTGATGGTGGCTATG7590.1330568182415814No Hit
AATTAGGCTGTGGGTGGTGATTAGTCGGTTGTTGATGAGATATTTGGAGG6330.1109683345809236No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTACCT100.0014760072245.000025
TCGTACC351.2063538E-8175.04
GGCTGTA1100.0167.045466
ACGACGG150.00496631163.33333245
CGTACCC251.1644217E-4147.05
AGGCTGT25600.0140.68365
TTAGGCT30850.0125.8752063
ATCTTAC13550.0124.7601553
GTCAATT17450.0124.255026
ATTAGGC31650.0124.241712
GCAGGTC17850.0122.156872
CTTACCC14600.0117.465755
CAGGTCA18700.0117.259353
TAGTCTT13300.0114.210528
TCTTACC16450.0104.255324
GCTGTGG31900.0104.06747
AATTTCA21650.0101.847589
TAGGCTG38800.096.610824
ACCCCGC18350.096.130798
AATTAGG42950.094.976721
Produced by FastQC (version 0.11.5)