FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005444614

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005444614
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences569033
Sequences flagged as poor quality0
Sequence length251
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAATCTTACCCCGCCTGTTTACCAAAAACATCACCTCTAGCATCACCAGT76841.3503610511165434No Hit
GGCAGGTCAATTTCACTGGTTAAAAGTAAGAGACAGCTGAACCCTCGTGG76781.349306630722647No Hit
GCCATACTAGTCTTTGCCGCCTGCGAAGCAGCGGTGGGCCTAGCCCTACT68121.197118620536946No Hit
AATTAGGCTGTGGGTGGTTGTGTTGATTCAAATTATGTGTTTTTTGGAAA58901.0350893533415462No Hit
AATTAGGCTGTGGGTAGAAGTAGAGGTTAAGGAGGGTGATGGTGGCTATG11790.20719360740062528No Hit
AATTAGGCTGTGGGTGGTGATTAGTCGGTTGTTGATGAGATATTTGGAGG7070.12424586974744874No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTACC401.2551027E-10183.750024
CGTACCC452.8376235E-10163.333345
AGGCTGT22000.0124.7272645
GCAGGTC15800.0120.949362
ATTAGGC24750.0116.808082
CAGGTCA16550.0115.4682853
GTCAATT16250.0114.584626
ATCTTAC17750.0113.18313
GTACCCC653.6689016E-9113.076936
CTTACCC19200.0107.18755
TTAGGCT27100.0105.3228763
TAGTCTT14750.0104.6440668
TGAGCAT7550.0103.841064245
GCTGTGG28600.092.517487
GGGTTAT13150.092.224335245
ACCCCGC23250.091.677428
TCTTACC22400.089.68754
AATTTCA20450.089.254279
GGCAGGT23950.085.4175341
TACCCCG24900.084.12657
Produced by FastQC (version 0.11.5)