FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005444618

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005444618
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences354331
Sequences flagged as poor quality0
Sequence length251
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AATTAGGCTGTGGGTGGTTGTGTTGATTCAAATTATGTGTTTTTTGGAAA68451.9318095227343925No Hit
AAATCTTACCCCGCCTGTTTACCAAAAACATCACCTCTAGCATCACCAGT60841.7170385882127164No Hit
GGCAGGTCAATTTCACTGGTTAAAAGTAAGAGACAGCTGAACCCTCGTGG29530.8334015369809584No Hit
GCCATACTAGTCTTTGCCGCCTGCGAAGCAGCGGTGGGCCTAGCCCTACT27540.7772393609365255No Hit
AATTAGGCTGTGGGTAGAAGTAGAGGTTAAGGAGGGTGATGGTGGCTATG6870.19388650724887183No Hit
AATTAGGCTGTGGGTGTGATAGGTGGCACGGAGAATTTTGGATTCTCAGG5220.14731987887032183No Hit
AATTAGGCTGTGGGTGGTGATGTAGAGGGTGATGGTAGATGTGGCGGGTT3600.10159991646229091No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGACC100.0014757719245.00002245
AGGCTGT16450.0195.851075
GCTGTGG19100.0166.753927
GCTCTGG150.004965521163.333337
TTAGGCT20400.0158.529423
ATCTTAC9600.0150.572923
ATTAGGC22850.0142.067832
CTTACCC10550.0139.33655
GGCTGTG23250.0136.989266
CTGTGGG23250.0136.462378
TAGGCTG24800.0130.403234
AATTAGG25900.0126.283781
GGGTTAT11250.0124.13334245
TAGTCTT7900.0102.3417748
GTCAATT8600.095.436056
TGTGGGT32750.095.381689
ACCCCGC15450.093.559878
CAGGTCA9050.090.690613
AGTCTTT9100.088.846159
TACCCCG16400.088.140247
Produced by FastQC (version 0.11.5)