FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005444619

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005444619
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences354331
Sequences flagged as poor quality0
Sequence length251
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AATTAGGCTGTGGGTGGTTGTGTTGATTCAAATTATGTGTTTTTTGGAAA64841.8299273842819284No Hit
AAATCTTACCCCGCCTGTTTACCAAAAACATCACCTCTAGCATCACCAGT60411.7049030426352763No Hit
GGCAGGTCAATTTCACTGGTTAAAAGTAAGAGACAGCTGAACCCTCGTGG31490.8887170470548724No Hit
GCCATACTAGTCTTTGCCGCCTGCGAAGCAGCGGTGGGCCTAGCCCTACT29070.8204193254329991No Hit
AATTAGGCTGTGGGTGTGATAGGTGGCACGGAGAATTTTGGATTCTCAGG4990.14082877309634212No Hit
AATTAGGCTGTGGGTAGAAGTAGAGGTTAAGGAGGGTGATGGTGGCTATG4390.1238954536859603No Hit
AATCTTACCCCGCCTGTTTACCAAAAACATCACCTCTAGCATCACCAGTA3890.10978435417730878No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GACACTT100.0014757719245.00002245
AGTGCCG100.0014757719245.00002245
TGCACTG100.0014757719245.000028
AGGCTGT16250.0170.369225
ATCTTAC8850.0168.870063
TTACCCG150.004965521163.333336
GCTGTGG17650.0149.915017
CTTACCC9950.0148.969855
TTAGGCT19800.0139.204543
TGCGAGG302.4059204E-4122.5245
ATTAGGC23200.0120.3879242
GGCTGTG22500.0118.688896
CTGTGGG22000.0118.602278
TCTTACC12550.0118.1075744
AATTAGG26000.0112.1346051
TAGGCTG24950.0111.943894
GGCTGTA953.6379788E-12103.157896
GGGTTAT11400.0102.083336245
ACCCCGC14550.0101.872858
CAGGTCA9050.0100.165753
Produced by FastQC (version 0.11.5)