FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005444620

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005444620
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences483600
Sequences flagged as poor quality0
Sequence length301
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCATACTAGTCTTTGCCGCCTGCGAAGCAGCGGTGGGCCTAGCCCTACT107252.217741935483871No Hit
GGCAGGTCAATTTCACTGGTTAAAAGTAAGAGACAGCTGAACCCTCGTGG93231.9278329197684037No Hit
AATTAGGCTGTGGGTGGTTGTGTTGATTCAAATTATGTGTTTTTTGGAAA58121.2018196856906533No Hit
AAATCTTACCCCGCCTGTTTACCAAAAACATCACCTCTAGCATCACCAGT56271.163564929693962No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGTCTT16100.0229.037268
GCGGTCG202.2813041E-5221.253
GGCGGTC202.2813041E-5221.252
GCAGGTC12750.0200.137252
GCCATAC22550.0165.487811
AGTCTTT22800.0162.379389
CAGGTCA15800.0160.569633
GTCAATT16550.0148.836876
GGTGCCA651.0077201E-9136.153851
AATTTCA18200.0135.343419
ATTAGGC17600.0126.54832
GGCAGGT21750.0117.321831
CTAGTCT33350.0110.569727
CCATACT34050.0110.4625552
AGGTCAA23450.0109.4456254
TCAATTT22550.0109.235047
AGGCTGT21100.0106.255935
TCTCGTT856.510163E-9104.117645295
GGTCAAT24250.0102.793815
ACCCCGC16700.0101.571858
Produced by FastQC (version 0.11.5)