FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005444621

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005444621
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences483600
Sequences flagged as poor quality0
Sequence length301
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCAGGTCAATTTCACTGGTTAAAAGTAAGAGACAGCTGAACCCTCGTGG98272.032051282051282No Hit
GCCATACTAGTCTTTGCCGCCTGCGAAGCAGCGGTGGGCCTAGCCCTACT87651.8124483043837882No Hit
AATTAGGCTGTGGGTGGTTGTGTTGATTCAAATTATGTGTTTTTTGGAAA63441.3118279569892473No Hit
AAATCTTACCCCGCCTGTTTACCAAAAACATCACCTCTAGCATCACCAGT49451.022539288668321No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGTCTT13600.0220.165448
GCAGGTC16650.0209.069082
CAGGTCA19350.0181.421193
GTCAATT20050.0173.615976
AATTTCA21800.0163.061949
AGTCTTT19450.0156.221089
GGCAGGT23550.0150.318471
GCCATAC20000.0149.71251
AGGTCAA25950.0135.279394
ATTAGGC19600.0132.448972
GGTCAAT26650.0131.726075
TCAATTT27100.0129.538767
AGGCTGT22900.0113.362455
CCATACT27200.0110.6252
CTAGTCT27100.0110.488947
CAATTTC32050.0110.4524158
ACCCCGC14100.0105.656038
TTAGGCT25800.0101.7635653
ATACTAG29950.0101.4524154
TACCCCG14450.0101.055377
Produced by FastQC (version 0.11.5)