FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005444622

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005444622
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences405446
Sequences flagged as poor quality0
Sequence length301
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCATACTAGTCTTTGCCGCCTGCGAAGCAGCGGTGGGCCTAGCCCTACT97562.4062390552625996No Hit
GGCAGGTCAATTTCACTGGTTAAAAGTAAGAGACAGCTGAACCCTCGTGG80621.9884275587871134No Hit
AAATCTTACCCCGCCTGTTTACCAAAAACATCACCTCTAGCATCACCAGT66021.6283302831943094No Hit
AATTAGGCTGTGGGTGGTTGTGTTGATTCAAATTATGTGTTTTTTGGAAA51401.2677397236623373No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGTCTT10800.0217.152778
GCAGGTC13550.0190.498152
GGTGCCA408.827556E-9184.374981
CAGGTCA15250.0170.22953
GTCAATT16350.0156.972476
CCGATTT200.0067360997147.49998295
GGCAGGT17950.0143.802231
AGTCTTT16550.0141.706959
AATTTCA18400.0139.48379
GCCATAC17450.0134.398281
TCTCGTT555.9135346E-8134.09091295
ATCTTAC15200.0132.944093
ATTAGGC13600.0131.231612
ACCCCGC16250.0124.353848
AGGCTGT14950.0119.381285
TCTTACC17600.0114.815344
TCAATTT22600.0113.561947
TACCCCG17850.0113.2072757
AGGTCAA22900.0112.7183464
GGTCAAT22850.0112.319475
Produced by FastQC (version 0.11.5)