FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005444624

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005444624
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences335872
Sequences flagged as poor quality0
Sequence length301
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCATACTAGTCTTTGCCGCCTGCGAAGCAGCGGTGGGCCTAGCCCTACT158884.730373475609756No Hit
AATTAGGCTGTGGGTGGTTGTGTTGATTCAAATTATGTGTTTTTTGGAAA136274.057200362042683No Hit
AAATCTTACCCCGCCTGTTTACCAAAAACATCACCTCTAGCATCACCAGT90632.6983493711890247No Hit
GGCAGGTCAATTTCACTGGTTAAAAGTAAGAGACAGCTGAACCCTCGTGG75182.238352705792683No Hit
AATTAGGCTGTGGGTGGTTGGCCATGGGTATGTTGTTAAGAAGAGGAATT9060.2697456173780488No Hit
AATTAGGCTGTGGGTGGTTGTATATAGCCTAGAATTTTTCGTTCGGTAAG4820.14350705030487804No Hit
AATTAGGCTGTGGGTGGTTGTGGGTATAATACTAAGTTGAGATGATATCA4260.12683403201219512No Hit
GGCAGGTCAATTTCACTGGTAGTAATATAATTGTTGGGACGATTAGTTTT3470.1033131669207317No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGGTCC157.235809E-6295.0295
GGTGCCA351.2732926E-11252.857131
TAGTCTT19250.0245.194818
TCTCGTT700.0231.7857295
AGGCTGT22350.0215.805365
AGTCTTT22200.0212.612619
GCCATAC23050.0206.052061
ATTAGGC24500.0198.071432
CGGATCC150.0028488024196.66667295
CCGGTCC150.0028488024196.66667295
GGACCTT150.0028488024196.66667295
GCTGTGG25900.0186.795367
ATCTTAC15400.0184.853913
TTAGGCT27700.0174.124563
TAGGCTG28450.0170.052724
GCAGGTC12750.0168.901962
ACCCCGC17300.0164.552028
CTGTGGG29600.0163.445948
TCTTACC17500.0162.671424
CCATACT29450.0161.77422
Produced by FastQC (version 0.11.5)