FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005444625

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005444625
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences335872
Sequences flagged as poor quality0
Sequence length301
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCATACTAGTCTTTGCCGCCTGCGAAGCAGCGGTGGGCCTAGCCCTACT143944.285561166158536No Hit
AATTAGGCTGTGGGTGGTTGTGTTGATTCAAATTATGTGTTTTTTGGAAA143094.26025390625No Hit
AAATCTTACCCCGCCTGTTTACCAAAAACATCACCTCTAGCATCACCAGT82512.456590605945122No Hit
GGCAGGTCAATTTCACTGGTTAAAAGTAAGAGACAGCTGAACCCTCGTGG78402.3342225609756095No Hit
AATTAGGCTGTGGGTGGTTGGCCATGGGTATGTTGTTAAGAAGAGGAATT8590.25575219131097565No Hit
AATTAGGCTGTGGGTGGTTGTATATAGCCTAGAATTTTTCGTTCGGTAAG5370.15988233612804878No Hit
AATTAGGCTGTGGGTGGTTGTGGGTATAATACTAAGTTGAGATGATATCA4140.12326124237804878No Hit
GCCATACTAGTCTTTGCCGCCTGCGAAACAGCGGTGGGCCTAGCCCTACT3680.10956554878048781No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGTCTT18350.0249.986378
AGGCTGT25450.0219.076635
AGTCTTT21300.0215.363869
ATTAGGC26000.0214.442322
GCCATAC21800.0207.71791
AAGGTCC150.0028488024196.66667295
TTAGGCT29200.0191.446913
GCTGTGG29600.0186.368247
GCAGGTC14500.0185.137922
ATCTTAC12350.0180.344133
CAGGTCA15300.0174.493453
GTCAATT15550.0173.585226
CCATACT26650.0169.915572
CTGTGGG32300.0168.506218
TAGGCTG33300.0167.875384
GGCTGTG33600.0165.059526
ACCCCGC14200.0156.848598
CATACTA28950.0156.416243
AATTAGG35900.0155.306411
AATTTCA17500.0153.49
Produced by FastQC (version 0.11.5)