FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005444627

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005444627
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences568523
Sequences flagged as poor quality0
Sequence length251
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AATTAGGCTGTGGGTGGTTGTGTTGATTCAAATTATGTGTTTTTTGGAAA95321.6766252200878418No Hit
AAATCTTACCCCGCCTGTTTACCAAAAACATCACCTCTAGCATCACCAGT91831.6152380818366188No Hit
GGCAGGTCAATTTCACTGGTTAAAAGTAAGAGACAGCTGAACCCTCGTGG47230.8307491517493576No Hit
GCCATACTAGTCTTTGCCGCCTGCGAAGCAGCGGTGGGCCTAGCCCTACT36370.6397278562168989No Hit
AATTAGGCTGTGGGTAGAAGTAGAGGTTAAGGAGGGTGATGGTGGCTATG8040.141419080670439No Hit
AATTAGGCTGTGGGTGTGATAGGTGGCACGGAGAATTTTGGATTCTCAGG7570.1331520448601024No Hit
AATTAGGCTGTAGGTAGAAGTAGAGGTTAAGGAGGGTGATGGTGGCTATG6590.11591439572365585No Hit
AATCTTACCCCGCCTGTTTACCAAAAACATCACCTCTAGCATCACCAGTA6270.11028577559746924No Hit
AATTAGGCTGTGGGTGGTGATTAGTCGGTTGTTGATGAGATATTTGGAGG5980.1051848386081126No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTACC500.0196.04
CGTACCC401.2551027E-10183.750025
ATCTTAC13900.0176.2593
AGGCTGT24050.0172.671525
ACGAGTC150.0049663056163.333333
TTACCCG150.0049663056163.333336
CTTACCC15100.0159.817895
TTAGGCT29700.0147.247483
AATTACG251.1644198E-4147.01
GCTGTGG26900.0145.724917
TACCTCG455.4114935E-8136.111117
GTACCCC551.1477823E-9133.636376
ATTAGGC35200.0126.676142
GGCTGTA1400.0122.499996
CTGTGGG33600.0115.5729148
TCTTACC21400.0114.485984
GGGTTAT18500.0113.22973245
TAGGCTG37250.0113.127514
GGCTGTG35200.0113.103696
TACCCCG23000.0109.184787
Produced by FastQC (version 0.11.5)